Molecular Histology Analysis of Cryopreserved Tissue Using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS)

Ibon Iloro; Iraide Escobés; Mikel Azkargorta; Félix Elortza

doi:10.1007/978-1-0716-1936-0_14

Molecular Histology Analysis of Cryopreserved Tissue Using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS)

Methods Mol Biol. 2022:2420:177-190. doi: 10.1007/978-1-0716-1936-0_14.

Authors

Ibon Iloro¹, Iraide Escobés¹, Mikel Azkargorta¹, Félix Elortza²

Affiliations

¹ CIC bioGUNE, Proteomics Platform, Basque Research & Technology Alliance (BRTA), ProteoRed-ISCIII, CIBERehd, Bizkaia Science and Technology Park, Derio, Spain.
² CIC bioGUNE, Proteomics Platform, Basque Research & Technology Alliance (BRTA), ProteoRed-ISCIII, CIBERehd, Bizkaia Science and Technology Park, Derio, Spain. felortza@cicbiogune.es.

PMID: 34905174
DOI: 10.1007/978-1-0716-1936-0_14

Abstract

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) has emerged as a powerful tool for analyzing the spatial distribution of peptides, small proteins, and other molecules within biological tissues. The obtained signals can be correlated with underlying tissue architecture, without any geometrical distortion, enabling the so-called molecular histology. Here, we analyzed cryopreserved tissue samples employing the MALDI-IMS for proteins and peptides. We used a nonstandard OCT-free cryo-slicing protocol, followed by Carnoy delipidation. Automated matrix spray was utilized to circumvent some of MALDI-IMS technology drawbacks in protein and peptide analysis.

Keywords: Imaging mass spectrometry; MALDI; MALDI-IMS; Molecular histology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Histological Techniques
Molecular Imaging
Peptides
Proteins
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

Peptides
Proteins