Paracoccus shandongensis sp. nov., Isolated from Activated Sludge

Abstract

A novel strain, wg2^T, was isolated from activated sludge obtained from wastewater treatment plant in Shandong province, China. The bacterium was Gram-strain-negative, aerobic, rod-shaped, non-flagellated and non-gliding. This bacterium was characterized to determine its taxonomic position using the polyphasic approach. Strain wg2^T grew at 25-45 °C (optimum, 30 °C), at salinities of 0-7.0% (w/v) NaCl (optimum, 0-2.0%) and at pH 7-9 (optimum, pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence showed that strain wg2^T clustered with species of genus Paracoccus and shares high similarities with Paracoccus sediminis DSM 26170 ^T (98.1%) and Paracoccus fontiphilus MVW-1 ^T (97.7%), respectively. The genome size of strain wg2^T was 3.93 Mbp and the DNA G + C content was 66.05%. The dDDH values and ANI between strain wg2^T and each of reference strains P. sediminis DSM 26170 ^T, P. fontiphilus MVW-1 ^T and P. denitrificans DSM 413 ^T were 18.3, 12.5, 24.5% and 85.3, 87.0, 78.4%, respectively. The major respiratory quinone was found to be Q-10 and the major fatty acid was C_18:1 ω7c. The polar lipids consisted of aminoglycolipid (AGL), phosphatidylcholine (PC), glycolipid (GL), phosphatidylserine (PS), phosphatidylglycerol phosphate (PGP), aminophospholipids (APL). Combining above descriptions, strain wg2^T should represent a novel species of genus Paracoccus, for which the name Paracoccus shandongensis sp. nov., is proposed. The type strain is wg2^T (= KCTC 72862 ^T = CCTCC AB 2019401 ^T).