Arrhythmogenic Hearts in PKD2 Mutant Mice Are Characterized by Cardiac Fibrosis, Systolic, and Diastolic Dysfunctions

Farideh Amirrad; Rajasekharreddy Pala; Kiumars Shamloo; Brian S Muntean; Surya M Nauli

doi:10.3389/fcvm.2021.772961

Arrhythmogenic Hearts in PKD2 Mutant Mice Are Characterized by Cardiac Fibrosis, Systolic, and Diastolic Dysfunctions

Front Cardiovasc Med. 2021 Nov 26:8:772961. doi: 10.3389/fcvm.2021.772961. eCollection 2021.

Authors

Farideh Amirrad^{1

2}, Rajasekharreddy Pala¹, Kiumars Shamloo¹, Brian S Muntean³, Surya M Nauli^{1

2}

Affiliations

¹ Department of Biomedical and Pharmaceutical Sciences, Chapman University, Irvine, CA, United States.
² Department of Medicine, University of California, Irvine, Orange, CA, United States.
³ Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, GA, United States.

Abstract

Autosomal dominant polycystic kidney disease (PKD) is a hereditary disorder affecting multiple organs, including the heart. PKD has been associated with many cardiac abnormalities including the arrhythmogenic remodeling in clinical evaluations. In our current study, we hypothesized that Pkd2 gene mutation results in structural and functional defects in the myocardium. The structural and functional changes of Pkd2 mutant hearts were analyzed in the myocardial-specific Pkd2 knockout (KO) mouse. We further assessed a potential role of TGF-b₁ signaling in the pathology of Pkd2-KO hearts. Hearts from age-matched 6-month-old MyH6•Pkd2 ^wt/wt (control or wild-type) and MyH6•Pkd2 ^flox/flox (mutant or Pkd2-KO) mice were used to study differential heart structure and function. Cardiac histology was used to study structure, and the "isolated working heart" system was adapted to mount and perfuse mouse heart to measure different cardiac parameters. We found that macrophage1 (M1) and macrophage 2 (M2) infiltration, transforming growth factor (TGF-b₁) and TGF-b₁ receptor expressions were significantly higher in Pkd2-KO, compared to wild-type hearts. The increase in the extracellular matrix in Pkd2-KO myocardium led to cardiac hypertrophy, interstitial and conduction system fibrosis, causing cardiac dysfunction with a predisposition to arrhythmia. Left ventricular (LV) expansion or compliance and LV filling were impaired in fibrotic Pkd2-KO hearts, resulted in diastolic dysfunction. LV systolic contractility and elastance decreased in fibrotic Pkd2-KO hearts, resulted in systolic dysfunction. Compared to wild-type hearts, Pkd2-KO hearts were less responsive to the pharmacological stress-test and changes in preload. In conclusion, Pkd2-KO mice had systolic and diastolic dysfunction with arrhythmogenic hearts.

Keywords: cardiac function; cardiovascular; fibrosis; inflammation; polycystic kidney disease.

Grants and funding

R01 HL147311/HL/NHLBI NIH HHS/United States