Cyclodextrin-dendrimers nanocomposites functionalized high performance liquid chromatography stationary phase for efficient separation of aromatic compounds

Shuxin Yu; Xuming Sha; Xiaoqian Zhou; Dandan Guo; Bowen Han; Shaohua Huang; Yan Zhu

doi:10.1016/j.chroma.2021.462730

Cyclodextrin-dendrimers nanocomposites functionalized high performance liquid chromatography stationary phase for efficient separation of aromatic compounds

J Chromatogr A. 2022 Jan 11:1662:462730. doi: 10.1016/j.chroma.2021.462730. Epub 2021 Dec 5.

Authors

Shuxin Yu¹, Xuming Sha², Xiaoqian Zhou², Dandan Guo³, Bowen Han², Shaohua Huang⁴, Yan Zhu⁵

Affiliations

¹ Institute of Drug Discovery and Technology, Ningbo University, Ningbo 315211, China; School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
² Institute of Drug Discovery and Technology, Ningbo University, Ningbo 315211, China.
³ Institute of Drug Discovery and Technology, Ningbo University, Ningbo 315211, China. Electronic address: guodandan@nbu.edu.cn.
⁴ Institute of Drug Discovery and Technology, Ningbo University, Ningbo 315211, China; Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo 315211, China. Electronic address: huangshaohua@nbu.edu.cn.
⁵ Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.

PMID: 34896734
DOI: 10.1016/j.chroma.2021.462730

Abstract

In this work, novel cyclodextrin-dendrimers nanocomposites functionalized high performance liquid chromatography stationary phases were developed for efficient separation of aromatic compounds. β-cyclodextrin was grafted onto the surface of silica gel matrix with poly (amidoamine) dendrimers as spacers. Scanning electron microscope, fourier transform infrared spectroscopy, element analysis and Brunner-Emmet-Teller measurement proved the successful grafting of cyclodextrin-dendrimers nanocomposites. The obtained stationary phases showed satisfactory separation effects for alkylbenzenes and benzenesulfonic acid substituents in reverse phase liquid chromatography mode. Weak hydrophilic and ion exchange interactions were also confirmed at the same time. Meanwhile, the effects of dendrimers and cyclodextrin on the chromatography performance were discussed. Separation mechanism of the stationary phases were verified by two-dimensional nuclear magnetic resonance technology, and the result implied that the proposed cyclodextrin-dendrimers nanocomposites functionalized stationary phases have significant prospects for separation and determination of more aromatic compounds in future.

Keywords: Aromatic compounds; Cyclodextrin; Dendrimers; Stationary phase.

MeSH terms

Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Cyclodextrins*
Dendrimers*
Hydrophobic and Hydrophilic Interactions
Nanocomposites*
Silicon Dioxide

Substances

Cyclodextrins
Dendrimers
Silicon Dioxide