Objective: The purpose of the present study was to clarify the expression of thyroid-stimulating hormone receptor (TSHR) in microglial cells, and to explore its function.
Materials and methods: Expression of TSHR in microglia was determined by Western blot, immunocytofluorescence and double immunohistofluorescence. Cyclic adenosine 3',5'-monophosphate (cAMP) production was measured after thyrotropin receptor stimulating antibody (TSAb) treatment.
Results: Results showed that TSHR protein was expressed and mainly located in the mouse microglia membrane. Moreover, TSAb stimulated cAMP production in mouse microglia (P<0.05).
Conclusions: This study demonstrated the presence of TSHR in microglial cells. Brain TSHR was able to respond specifically to TSAb stimulation, suggesting that TSHR expression is functional. As microglia are innate immune cells that maintain environmental stability in the central nervous system and play a key role in many neuroimmune diseases, expression of functional TSHR in microglia has important pathophysiological implications.
Keywords: Anticorps stimulant le récepteur de la thyrotropine; Auto-immune thyroid disease; Brain; Cerveau; Maladie thyroïdienne auto-immune; Microglia; Microglie; Récepteur de l’hormone stimulant la thyroïde; Thyroid-stimulating hormone receptor; Thyrotropin receptor stimulating antibody.
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