Highly sensitive and low-cost colourimetric detection of glucose and ascorbic acid based on silver nanozyme biosynthesized by Gleditsia australis fruit

Abstract

In this study, a simple, eco-friendly and low-cost approach was used to fabricate silver nanoparticles (AgNPs) from an aqueous extract of Gleditsia australis (GA) fruit. The nanoparticles synthesized in the optimal condition have an average size of 14 nm. The peroxidase-like activity of GA-AgNP in the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in combination with hydrogen peroxide (H₂O₂) was investigated. Further, optimal conditions for the use of peroxidase-like catalytic activity in sensing applications were identified. The colourimetric detection of H₂O₂ showed a linear range of 1-8 mM with a limit of detection (LOD) of 0.34 mM. The oxidation of TMB (red-TMB) enables the detection of glucose, which is converted into H₂O₂ and gluconic acid in the presence of the enzyme glucose oxidase. The observations showed linearity from 0.05 to 1.5 mM with a LOD of 0.038 mM. Moreover, the blue colour of oxidized TMB (ox-TMB) was reduced according to ascorbic acid (AA) concentration, with a linear range of 0.03-0.14 mM and a LOD of 3.0 μM. The practical use of the sensing system for the detection of AA was studied using real fruit juice and showed good sensitivity. Hence, the easy-to-use peroxidase-like sensor provides a new platform for the detection of bioactive compounds in biological systems.

Keywords: Ascorbic acid; Colourimetric detection; Glucose; Hydrogen peroxide; Nanozyme; Silver nanoparticles.