Osteogenic differentiation of human mesenchymal stem cells on substituted calcium phosphate/chitosan composite scaffold

Antonia Ressler; Maja Antunović; Laura Teruel-Biosca; Gloria Gallego Ferrer; Slaven Babić; Inga Urlić; Marica Ivanković; Hrvoje Ivanković

doi:10.1016/j.carbpol.2021.118883

Osteogenic differentiation of human mesenchymal stem cells on substituted calcium phosphate/chitosan composite scaffold

Carbohydr Polym. 2022 Feb 1:277:118883. doi: 10.1016/j.carbpol.2021.118883. Epub 2021 Nov 10.

Authors

Antonia Ressler¹, Maja Antunović², Laura Teruel-Biosca³, Gloria Gallego Ferrer⁴, Slaven Babić⁵, Inga Urlić⁶, Marica Ivanković⁷, Hrvoje Ivanković⁸

Affiliations

¹ Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, p.p.177, 10 000 Zagreb, Croatia. Electronic address: aressler@fkit.hr.
² Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, p.p.177, 10 000 Zagreb, Croatia.
³ Centre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain. Electronic address: lautebio@doctor.upv.es.
⁴ Centre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain; Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 46022 Valencia, Spain. Electronic address: ggallego@ter.upv.es.
⁵ UHC "Sestre Milosrdnice", Department for Traumatology, Draškovićeva 19, 10 000 Zagreb, Croatia.
⁶ Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb 10 000, Croatia. Electronic address: ingam@biol.pmf.hr.
⁷ Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, p.p.177, 10 000 Zagreb, Croatia. Electronic address: mivank@fkit.hr.
⁸ Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, p.p.177, 10 000 Zagreb, Croatia. Electronic address: hivan@fkit.hr.

PMID: 34893286
DOI: 10.1016/j.carbpol.2021.118883

Abstract

Ionic substitutions are a promising strategy to enhance the biological performance of calcium phosphates (CaP) and composite materials for bone tissue engineering applications. However, systematic studies have not been performed on multi-substituted organic/inorganic scaffolds. In this work, highly porous composite scaffolds based on CaPs substituted with Sr²⁺, Mg²⁺, Zn²⁺ and SeO₃^2- ions, and chitosan have been prepared by freeze-gelation technique. The scaffolds have shown highly porous structure, with very well interconnected pores and homogeneously dispersed CaPs, and high stability during 28 days in the degradation medium. Osteogenic potential of human mesenchymal stem cells seeded on scaffolds has been determined by histological, immunohistochemical and RT-qPCR analysis of cultured cells in static and dynamic conditions. Results indicated that ionic substitutions have a beneficial effect on cells and tissues. The scaffolds with multi-substituted CaPs have shown increased expression of osteogenesis related markers and increased phosphate deposits, compared to the scaffolds with non-substituted CaPs.

Keywords: Chitosan; Hydroxyapatite; Ionic substitution; Osteogenic differentiation; Perfusion-bioreactor; Scaffolds.

MeSH terms

Calcium Phosphates / chemistry
Calcium Phosphates / pharmacology*
Cell Differentiation / drug effects
Cells, Cultured
Chitosan / chemistry
Chitosan / pharmacology*
Humans
Mesenchymal Stem Cells / drug effects*
Osteogenesis / drug effects*
Tissue Engineering
Tissue Scaffolds / chemistry*

Substances

Calcium Phosphates
Chitosan
calcium phosphate