Protective Effects of Mogroside V on Oxidative Stress Induced by H2O2 in Skin Fibroblasts

Drug Des Devel Ther. 2021 Dec 1:15:4901-4909. doi: 10.2147/DDDT.S337524. eCollection 2021.

Abstract

Purpose: Damage caused by oxidative stress leads to the premature aging of cells. Mogrosides, the main active components of Siraitia grosvenorii, have strong antioxidant activity; however, it is unclear whether mogroside V (MV) exerts these effects in skin cells. This was investigated in the present study by evaluating the protective effects of MV against oxidative damage induced by hydrogen peroxide (H2O2) in skin fibroblasts.

Methods: Mouse skin fibroblasts (MSFs) were treated with H2O2 and cell viability, total antioxidant capacity, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, and antioxidant enzyme activity were assessed.

Results: Treatment with MV reduced the ROS level and MDA content in MSFs treated with H2O2. This was accompanied by increased superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities.

Conclusion: MV reduces H2O2-induced oxidative stress and enhances endogenous antioxidant activity in skin fibroblasts. Thus, MV can potentially be used as an ingredient in anti-aging cosmetic products.

Keywords: Siraitia grosvenorii; antioxidant; mogroside V; oxidative stress.

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Hydrogen Peroxide / antagonists & inhibitors*
  • Hydrogen Peroxide / pharmacology
  • Mice
  • Oxidative Stress / drug effects
  • Protective Agents / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Skin / drug effects*
  • Skin / metabolism
  • Triterpenes / pharmacology*

Substances

  • Antioxidants
  • Protective Agents
  • Reactive Oxygen Species
  • Triterpenes
  • mogroside V
  • Hydrogen Peroxide