Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species

Yanpeng Cheng; Wenjing Lei; Xiaoxia Wang; Zhi Tian; Haican Liu; Jing Yang; Shan Lu; Xin-He Lai; Ji Pu; Ying Huang; Sihui Zhang; Caixin Yang; Xingxing Lian; Yibo Bai; Kanglin Wan; Suping Wang; Jianguo Xu

doi:10.1099/ijsem.0.005019

Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species

Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005019.

Authors

Yanpeng Cheng^{1

2}, Wenjing Lei^{1

2}, Xiaoxia Wang², Zhi Tian^{1

2}, Haican Liu², Jing Yang^{2

3

4}, Shan Lu^{2

3

4}, Xin-He Lai⁵, Ji Pu², Ying Huang², Sihui Zhang^{2

6}, Caixin Yang^{1

2}, Xingxing Lian^{1

2}, Yibo Bai^{1

2}, Kanglin Wan², Suping Wang¹, Jianguo Xu^{1

2

3

4

6}

Affiliations

¹ Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.
² State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
³ Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai, 201508, PR China.
⁴ Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, PR China.
⁵ Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, Henan Province, PR China.
⁶ Department of Laboratorial Science and Technology & Vaccine Research Center, School of Public Health, Peking University, Beijing, 100191, PR China.

PMID: 34878372
DOI: 10.1099/ijsem.0.005019

Abstract

Four bacterial strains (LJ126^T/S18 and Z-34^T/S20) recovered from faecal samples of Tibetan antelopes on the Qinghai-Tibet Plateau of China were analysed using a polyphasic approach. All four isolates were aerobic, short rod-shaped, non-motile, Gram-stain-positive, acid-fast and fast-growing. Phylogenetic analyses based upon 16S rRNA and whole-genome sequences showed that the two pair of strains formed two distinct branches within the evolutionary radiation of the genus Mycolicibacterium. Strains LJ126^T/S18 and Z-34^T/S20 were most closely related to Mycolicibacterium austroafricanum CCUG 37667^T, Mycobacterium aurum NCTC 10437^T, Mycobacterium pyrenivorans DSM 44605^T, Mycobacterium monacense JCM 15658^T, Mycolicibacterium sarraceniae JCM 30395^T, Mycolicibacterium tokaiense JCM 6373^T and Mycobacterium murale JCM 13392^T, but readily distinguished from the known species by a combination of chemotaxonomic and phenotypic features and by low average nucleotide identity values (74.4-84.9 %). Consequently, the two strain pairs are considered to represent different novel species of Mycolicibacterium for which the names Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov. are proposed, with LJ126^T (=CGMCC 1.1992^T=KCTC 49535^T) and Z-34^T (=CGMCC 1.1993^T=DSM 106172^T) as the respective type strains.

Keywords: Mycolicibacterium; Qinghai–Tibet Plateau; polyphasic taxonomy.

MeSH terms

Animals
Antelopes / microbiology*
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Feces / microbiology
Mycobacteriaceae / classification*
Mycobacteriaceae / isolation & purification
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Tibet

Substances

DNA, Bacterial
RNA, Ribosomal, 16S