Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)

STAR Protoc. 2022 Mar 18;3(1):101051. doi: 10.1016/j.xpro.2021.101051. Epub 2021 Dec 3.

Abstract

Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).

Keywords: Clinical Protocol; Health Sciences; Immunology; Mass Spectrometry; Metabolomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • COVID-19 / diagnosis*
  • COVID-19 / metabolism
  • Chromatography, High Pressure Liquid / methods*
  • Computational Biology
  • Diagnostic Tests, Routine
  • Gene Expression Profiling
  • Genetic Techniques
  • Humans
  • Influenza A Virus, H1N1 Subtype / metabolism
  • Influenza A Virus, H1N1 Subtype / pathogenicity
  • Mass Spectrometry / methods
  • Metabolome
  • Metabolomics / methods*
  • Reference Standards
  • SARS-CoV-2 / metabolism
  • SARS-CoV-2 / pathogenicity
  • Specimen Handling / methods