Immobilization and Characterization of Pectinase onto the Cationic Polystyrene Resin

Qingxian Miao; Chen Zhang; Shuai Zhou; Lingchao Meng; Liulian Huang; Yonghao Ni; Lihui Chen

doi:10.1021/acsomega.1c04374

Immobilization and Characterization of Pectinase onto the Cationic Polystyrene Resin

ACS Omega. 2021 Nov 18;6(47):31683-31688. doi: 10.1021/acsomega.1c04374. eCollection 2021 Nov 30.

Authors

Qingxian Miao^{1

2}, Chen Zhang¹, Shuai Zhou¹, Lingchao Meng¹, Liulian Huang¹, Yonghao Ni^{1

3}, Lihui Chen¹

Affiliations

¹ College of Material Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
² State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
³ Limerick Pulp and Paper Centre, Department of Chemical Engineering, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada.

Abstract

In the present study, the immobilization of free pectinase onto polystyrene resin beads via crosslinking with glutaraldehyde was investigated. The immobilized pectinase was characterized by Fourier transform infrared spectroscopy and confocal laser scanning microscopy. After optimizing the immobilization conditions, the optimum pH of immobilized pectinase shifted from 8.0 to 8.5 and the optimum temperature shifted from 45 to 60 °C, showing its improved stability to temperature and pH compared with the free pectinase. The Michaelis-Menten constant K _m value of free and immobilized pectinase was determined to be 1.95 and 5.36 mM, respectively. The storage stability of immobilized pectinase was demonstrated with 36.8% of the initial activity preserved after 30 days at 25 °C. The reusability of the immobilized pectinase activity was 54.6% of its initial activity after being recycled six times. Therefore, based on the findings mentioned above, it can be inferred that this simple immobilization technique for pectinase appears to be promising for industrial applications.