The complete mitochondrial genome of Rana johnsi (Smith, 2009) (Anura: Ranidae) and its phylogeny

Qing-Ping Chen; Lian Wu; Shu-Sheng Zhang; Lei-Lei Liu; Wan-Ting Jin; Jia-Yong Zhang; Yong-Pu Zhang; Dan-Na Yu

doi:10.1080/23802359.2021.2002213

The complete mitochondrial genome of Rana johnsi (Smith, 2009) (Anura: Ranidae) and its phylogeny

Mitochondrial DNA B Resour. 2021 Nov 29;6(12):3493-3495. doi: 10.1080/23802359.2021.2002213. eCollection 2021.

Authors

Qing-Ping Chen^{1

2}, Lian Wu¹, Shu-Sheng Zhang³, Lei-Lei Liu³, Wan-Ting Jin¹, Jia-Yong Zhang^{1

4}, Yong-Pu Zhang², Dan-Na Yu^{1

4}

Affiliations

¹ College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, PR China.
² College of Life and Environmental Science, Wenzhou University, Wenzhou, PR China.
³ The Management Center of Wuyanling, National Natural Reserve in Zhejiang, Wenzhou, PR China.
⁴ Key Lab of Wildlife Biotechnology, Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua, PR China.

Abstract

Rana johnsi (Smith 2009) firstly considered as the member of genus Pseudorana, has been moved into the genus Rana. In this study, we sequenced the complete mitochondrial (mt) genome of R. johnsi using the Sanger method. The circular mt genome was 17,873 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosome RNA genes, and one control region. The overall nucleotide composition in majority-strand was 28% A, 29% T, 29% C, and 14% G. We discussed the phylogenetic relationship of R. johnsi in genus Rana using ML and BI analyses based on 13 PCGs. Excluding the clade of subgenus Lithobates, Rana draytonii was the basal clade to all other Rana species, which included R. johnsi as the basal clade. The monophyly of genus Rana was supported, whereas Pseudorana was failed to support.

Keywords: Rana johnsi; mitogenome; phylogeny.

Grants and funding

This work was supported by the College Students’ Innovation and Entrepreneurship Project of China (201910345030 and 202010345026) and the College Students’ Innovation and Entrepreneurship Project of Zhejiang Province (202010345R128). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.