Identification and Characterization of a Novel Chromosomal Aminoglycoside 2'- N-Acetyltransferase, AAC(2')-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33

Kexin Zhou; Jialei Liang; Xu Dong; Peiyao Zhang; Chunlin Feng; Weina Shi; Mengdi Gao; Qiaoling Li; Xueya Zhang; Junwan Lu; Xi Lin; Kewei Li; Hailin Zhang; Mei Zhu; Qiyu Bao

doi:10.3389/fmicb.2021.711037

Identification and Characterization of a Novel Chromosomal Aminoglycoside 2'- N-Acetyltransferase, AAC(2')-If, From an Isolate of a Novel Providencia Species, Providencia wenzhouensis R33

Front Microbiol. 2021 Nov 19:12:711037. doi: 10.3389/fmicb.2021.711037. eCollection 2021.

Authors

Kexin Zhou^{1

2

3}, Jialei Liang^{1

2

3}, Xu Dong^{2

3}, Peiyao Zhang^{2

3}, Chunlin Feng^{2

3}, Weina Shi^{2

3}, Mengdi Gao^{2

3}, Qiaoling Li^{1

2

3}, Xueya Zhang^{1

2

3}, Junwan Lu^{2

3}, Xi Lin^{2

3}, Kewei Li^{2

3}, Hailin Zhang^{1

3}, Mei Zhu⁴, Qiyu Bao^{1

2

3}

Affiliations

¹ The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.
² Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
³ Institute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
⁴ Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou, China.

Abstract

Multidrug-resistant bacteria from different sources have been steadily emerging, and an increasing number of resistance mechanisms are being uncovered. In this work, we characterized a novel resistance gene named aac(2')-If from an isolate of a novel Providencia species, Providencia wenzhouensis R33 (CCTCC AB 2021339). Susceptibility testing and enzyme kinetic parameter analysis were conducted to determine the function of the aminoglycoside 2'-N-acetyltransferase. Whole-genome sequencing and comparative genomic analysis were performed to elucidate the molecular characteristics of the genome and the genetic context of the resistance gene-related sequences. Among the functionally characterized resistance genes, AAC(2')-If shares the highest amino acid sequence identity of 70.79% with AAC(2')-Ia. AAC(2')-If confers resistance to several aminoglycoside antibiotics, showing the highest resistance activity against ribostamycin and neomycin. The recombinant strain harboring aac(2')-If (pUCP20-aac(2')-If/DH5α) showed 256- and 128-fold increases in the minimum inhibitory concentration (MIC) levels to ribostamycin and neomycin, respectively, compared with those of the control strains (DH5α and pUCP20/DH5α). The results of the kinetic analysis of AAC(2')-If were consistent with the MIC results of the cloned aac(2')-If with the highest catalytic efficiency for ribostamycin (k _cat /K _m ratio = [3.72 ± 0.52] × 10⁴ M^-1 ^⋅s^-1). Whole-genome sequencing demonstrated that the aac(2')-If gene was located on the chromosome with a relatively unique genetic environment. Identification of a novel aminoglycoside resistance gene in a strain of a novel Providencia species will help us find ways to elucidate the complexity of resistance mechanisms in the microbial population.

Keywords: AAC(2cpsdummy′); Providencia; aminoglycoside; kinetic analysis; resistance.