Combined effects of polystyrene microplastics and copper on antioxidant capacity, immune response and intestinal microbiota of Nile tilapia (Oreochromis niloticus)

Abstract

Microplastics (MPs) coexist with other pollutants (such as heavy metals) in water, adversely impacting aquatic organisms, which might cause unpredictable ecological risks. This study aims to evaluate the effect of copper (Cu²⁺) and polystyrene microplastics (PS-MPs) on antioxidant capacity, immune response and intestinal microbiota of Nile tilapia. Cu²⁺ and PS-MPs co-exposure enhanced Cu²⁺ bioaccumulation in the liver of fish compared with Cu²⁺-alone exposure. Fish exposed to PS-MPs and Cu²⁺ displayed histopathologic alterations in the liver, intestine and gill. Exposure at low concentrations of Cu²⁺ in the C0 and CP0 groups can improve antioxidant capacity and immune response, while oxidative damage and inflammation existed in the high concentration of Cu²⁺ groups. Intestinal microbiota results showed that the diversity and structure were changed by Cu²⁺ and PS-MPs exposure, and harmful bacterium even increased at high concentration of Cu²⁺ and PS-MPs exposure groups. All in all, PS-MPs aggravate the accumulation of Cu²⁺ and lead to perturbations in biological systems of Nile tilapia.

Keywords: Antioxidant capacity; Copper; Immune response; Intestinal microbiota; Nile tilapia; Polystyrene microplastics.