Biodegradation of λ-cyhalothrin through cell surface display of bacterial carboxylesterase

Abstract

Pyrethroids are the third widespread used insecticides globally which have been extensively applied in agricultural or household environments. Due to continuous applications, pyrethroids have been detected both in living cells and environments. The permanent exposure to pyrethroids have caused substantial health risks and ecosystem concerns. In this work, a λ-cyhalothrin (one kind of pyrethroid insecticides) degrading bacterium Bacillus velezensis sd was isolated and a carboxylesterase gene, CarCB2 was characterized. A whole cell biocatalyst was developed for λ-cyhalothrin biodegradation by displaying CarCB2 on the surface of Escherichia coli cells. CarCB2 was successfully displayed and functionally expressed on E. coli cells with optimal pH and temperature of 7.5 and 30 °C, using p-NPC₄ as substrate, respectively. The whole cell biocatalyst exhibited better stability than the purified CarCB2, and approximately 120%, 60% or 50% of its original activity at 4 °C, 30 °C or 37 °C over a period of 35 d was retained, respectively. No enzymatic activity was detected when incubated the purified CarCB2 at 30 °C for 120 h, or 37 °C for 72 h, respectively. Additionally, 30 mg/L of λ-cyhalothrin was degraded in citrate-phosphate buffer by 10 U of the whole cell biocatalyst in 150 min. This work reveals that the whole cell biocatalyst affords a promising approach for efficient biodegradation of λ-cyhalothrin, and might have the potential to be applied in further environmental bioremediation of other different kinds of pyrethroid insecticides.

Keywords: Bacillus velezensis sd; Carboxylesterase; Surface display; Whole-cell biocatalyst; λ-cyhalothrin.