CRISPR/Cas Technologies and Their Applications in Escherichia coli

Huina Dong; Yali Cui; Dawei Zhang

doi:10.3389/fbioe.2021.762676

CRISPR/Cas Technologies and Their Applications in Escherichia coli

Front Bioeng Biotechnol. 2021 Nov 11:9:762676. doi: 10.3389/fbioe.2021.762676. eCollection 2021.

Authors

Huina Dong^{1

2}, Yali Cui^{1

2}, Dawei Zhang^{1

2

3}

Affiliations

¹ Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
² Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
³ University of Chinese Academy of Sciences, Beijing, China.

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. However, these systems unfortunately have not been developed and applied in bacteria as extensively as in eukaryotic organism. Here, the research progress on the most widely used CRISPR/Cas tools and their applications in Escherichia coli is summarized. Genome editing based on homologous recombination, non-homologous DNA end-joining, transposons, and base editors are discussed. Finally, the state of the art of transcriptional regulation using CRISPRi is briefly reviewed. This review provides a useful reference for the application of CRISPR/Cas systems in other bacterial species.

Keywords: CRISPR system; Escherichia coli; knock-down; knock-in; transcriptional regulation.

Publication types

Review