EasyCatch, a convenient, sensitive and specific CRISPR detection system for cancer gene mutations

Yin Liu; Yanling Chen; Lu Dang; Yixin Liu; Shisheng Huang; Sanyun Wu; Peixiang Ma; Hongqiang Jiang; Yi Li; Yunbao Pan; Yongchang Wei; Xiaodong Ma; Ming Liu; Quanjiang Ji; Tian Chi; Xingxu Huang; Xinjie Wang; Fuling Zhou

doi:10.1186/s12943-021-01456-x

EasyCatch, a convenient, sensitive and specific CRISPR detection system for cancer gene mutations

Mol Cancer. 2021 Dec 2;20(1):157. doi: 10.1186/s12943-021-01456-x.

Authors

Yin Liu¹, Yanling Chen¹, Lu Dang², Yixin Liu³, Shisheng Huang⁴, Sanyun Wu¹, Peixiang Ma⁵, Hongqiang Jiang¹, Yi Li³, Yunbao Pan⁶, Yongchang Wei³, Xiaodong Ma⁷, Ming Liu⁸, Quanjiang Ji⁹, Tian Chi⁴, Xingxu Huang¹⁰, Xinjie Wang¹¹, Fuling Zhou¹²

Affiliations

¹ Department of Hematology, Zhongnan Hospital of Wuhan University, No.169 Donghu Road, Wuhan, 430072, China.
² Department of Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
³ Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, No.169 Donghu Road, Wuhan, 430072, China.
⁴ Gene Editing Center, School of Life Science and Technology, ShanghaiTech University, 100 Haike Rd., Pudong New Area, Shanghai, 201210, China.
⁵ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China.
⁶ Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, 430072, China.
⁷ Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, Center for Studies of Psychological Application, South China Normal University, Guangzhou, 510631, China.
⁸ State Key Laboratory of Respiratory Disease/National Clinical Research Center for Respiratory Disease/National Center for Respiratory Medicine/Guangzhou Institute of Respiratory Health/The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, China.
⁹ School of Physical Science and Technology, ShanghaiTech University, 100 Haike Rd., Pudong New Area, Shanghai, 201210, China.
¹⁰ Gene Editing Center, School of Life Science and Technology, ShanghaiTech University, 100 Haike Rd., Pudong New Area, Shanghai, 201210, China. huangxx@shanghaitech.edu.cn.
¹¹ Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. wang_xin_jie@126.com.
¹² Department of Hematology, Zhongnan Hospital of Wuhan University, No.169 Donghu Road, Wuhan, 430072, China. zhoufuling@whu.edu.cn.

No abstract available

Keywords: CRISPR detection; Drug resistance; Leukemia; SNP detection; cancer mutation.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats*
Genes, Neoplasm*
Genetic Testing / methods*
Genetic Testing / standards
Humans
Mutation
Neoplasms / diagnosis*
Neoplasms / genetics*
Reproducibility of Results
Sensitivity and Specificity

Publication types

MeSH terms

Grants and funding