Synthesis of a covalent organic framework with hydrazine linkages and its application in open-tubular capillary electrochromatography

Abstract

Recently, covalent organic frameworks (COFs), owning to their excellent and unique properties, are attracting the attention of numerous researchers in some areas, especially the domain of chromatographic separation. However, the application of hydrazine linkages COFs in open-tubular capillary electrochromatography (OT-CEC) lies in the early stage at present. Herein, a well-crystallized hydrazine-linked COF (Tf-DHzOH) was synthesized successfully from 2,5-dihydroxyterephtalohydrazide and 1,3,5-triformyl-benzene. Tf-DHzOH was firstly regarded as a stationary phase material to prepare the Tf-DHzOH coated capillaries with different coating thickness by covalent bonding in this work. The characterization results showed that Tf-DHzOH was successfully synthesized. The separation performance and stability of the Tf-DHzOH coated capillary were evaluated by considering amino acids, sulfonamides, tetracyclines and benzene compounds as analytes. The relative standard deviations (RSDs) of separation time in the intra-day (n = 9), inter-day (n = 6), column-to-column (n = 3) and batch-to-batch (n = 3) were 0.76-4.97%, 1.59-5.94%1.78-8.72% and 1.66%-8.23%, respectively, the RSDs of peak areas were 1.90-5.16%, 1.73-5.24%, 1.26-7.33% and 3.77%-11.24%, respectively. It was confirmed that there was no visible change of separation efficiency after the Tf-DHzOH-coated capillary was used more than 200 runs. The results make clear that 2D hydrazine-linked COF (Tf-DHzOH) has superior potential as the stationary phase in OT-CEC for chromatographic separation.

Keywords: Amino acids; Benzene compounds; Capillary electrochromatography; Hydrazine-linked COF; Sulfonamides; Tetracyclines; Tf-DHzOH.