Toxicity of selenium nanoparticles on Poterioochromonas malhamensis algae in Waris-H culture medium and Lake Geneva water: Effect of nanoparticle coating, dissolution, and aggregation

Abstract

Understanding the algal toxicity of selenium nanoparticles (SeNPs) in aquatic systems by considering SeNPs physicochemical properties and environmental media characteristics is a concern of high importance for the evaluation and prediction of risk assessment. In this study, chitosan (CS) and sodium carboxymethyl cellulose (CMC) coated SeNPs are considered using Lake Geneva water and a Waris-H cell culture medium to investigate the effect of SeNPs on the toxicity of algae Poterioochromonas malhamensis, a widespread mixotrophic flagellate. The influence of surface coating, z-average diameters, ζ-potentials, aggregation behavior, ions release, and medium properties on the toxicity of SeNPs to algae P. malhamensi was investigated. It is found that SeNPs are 5-10 times more toxic in Lake Geneva water compared to the culture medium, suggesting that the traditional algal tests in Waris-H culture medium currently underestimate the toxicity of NPs in a natural water environment. Despite significant dissolution, it is also found that SeNPs themselves are the toxicity driver, and dissolved ions have only a marginal influence on toxicity. SeNPs diameter is found a minor factor in toxicity. Based on a principal component analysis (PCA) it is found that in Lake Geneva water, the nature of the surface coating (CMC versus CS) is the most influential factor controlling the toxicity of SeNPs. In the culture medium, surface coating, ζ-potential, and aggregation are found to contribute at the same level. These results highlight the importance of considering in details both NPs intrinsic and media properties in the evaluation of NPs biological effects.

Keywords: Algae; Ecotoxicity; Principal component analysis; Selenium nanoparticles; Stability; Surface coating.