Preparing Ion Channel Switch Membrane-Based Biosensors

Amani Alghalayini; Charles G Cranfield; Bruce A Cornell; Stella M Valenzuela

doi:10.1007/978-1-0716-1843-1_2

Preparing Ion Channel Switch Membrane-Based Biosensors

Methods Mol Biol. 2022:2402:13-20. doi: 10.1007/978-1-0716-1843-1_2.

Authors

Amani Alghalayini^{1

2}, Charles G Cranfield^{3

4}, Bruce A Cornell^{4

5}, Stella M Valenzuela^{3

4

6}

Affiliations

¹ School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia. amani.alghalayini@uts.edu.au.
² ARC Research Hub for Integrated Devices for End-user Analysis at Low-levels (IDEAL), Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia. amani.alghalayini@uts.edu.au.
³ School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia.
⁴ ARC Research Hub for Integrated Devices for End-user Analysis at Low-levels (IDEAL), Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
⁵ Surgical Diagnostics Pty Ltd., Roseville, NSW, Australia.
⁶ Institute for Biomedical Materials and Devices, University of Technology Sydney, Sydney, NSW, Australia.

PMID: 34854032
DOI: 10.1007/978-1-0716-1843-1_2

Abstract

Monitoring the changes in membrane conductance using electrical impedance spectroscopy is the platform of membrane-based biosensors in order to detect a specific target molecule. These biosensors represent the amalgamation of an electrical conductor such as gold and a chemically tethered bilayer lipid membrane with specific incorporated ion channels such as gramicidin-A that is further functionalized with detector molecules of interest.

Keywords: Biosensor; Electrical impedance spectroscopy; Gramicidin-A; Ion channels; Tethered bilayer lipid membranes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Dielectric Spectroscopy
Gramicidin
Ion Channels
Lipid Bilayers

Substances

Ion Channels
Lipid Bilayers
Gramicidin