Embryonic expression patterns of TBC1D10 subfamily genes in zebrafish

Shuna Sun; Ziyin Liu; Qiu Jiang; Yunzeng Zou

doi:10.1016/j.gep.2021.119226

Embryonic expression patterns of TBC1D10 subfamily genes in zebrafish

Gene Expr Patterns. 2022 Mar:43:119226. doi: 10.1016/j.gep.2021.119226. Epub 2021 Nov 27.

Authors

Shuna Sun¹, Ziyin Liu², Qiu Jiang³, Yunzeng Zou⁴

Affiliations

¹ Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, 201102, PR China.
² Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai, 200032, PR China.
³ Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai, 200032, PR China. Electronic address: jiang.qiu@zs-hospital.sh.cn.
⁴ Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai, 200032, PR China. Electronic address: zou.yunzeng@zs-hospital.sh.cn.

PMID: 34843939
DOI: 10.1016/j.gep.2021.119226

Abstract

TBC1D10 subfamily has three members TBC1D10A-C, with the physiological and pathological functions such as melanosome transport, exosome secretion, and T-cell activation. However, the gene expression patterns and functions of this subfamily during embryonic development remain mysterious. In this study, we took advantage of zebrafish model to elucidate the spatial and temporal expression patterns of TBC1D10 subfamily genes including tbc1d10aa, tbc1d10ab, tbc1d10b, and tbc1d10c. Whole-mount in situ hybridization results showed robust tbc1d10aa expression and faint tbc1d10b expression as maternal transcripts except tbc1d10ab and tbc1d10c. In addition to pectoral fins, otic vesicles, and pharyngeal arch tissues, varying degrees of expression of all four subfamily members were observed in brain tissues and eyes (retinal inner nuclear layer). Besides, tbc1d10ab exhibited unique and enriched expression in the developing liver. Despite genetic conservativeness, all four members of zebrafish TBC1D10 subfamily shared several similarities and exhibited some distinctions in the expression patterns, indicating that they might have different and exclusive functions to be further explored.

Keywords: Brain; GTPase-activation protein; Liver; Retinal inner nuclear layer; TBC1D10; Zebrafish.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Embryonic Development / genetics
Gene Expression Regulation, Developmental
In Situ Hybridization
Zebrafish Proteins* / genetics
Zebrafish Proteins* / metabolism
Zebrafish*

Substances

Zebrafish Proteins