The stimulatory effect of elevated CO2 on soil respiration is unaffected by N addition

Abstract

Global atmospheric CO₂ keeps rising and brings about significant effects on ecosystem carbon (C) cycling by altering C processes in soils. Soil C responses to elevated CO₂ are highly uncertain, and how elevated CO₂ interacts with other factors, such as nitrogen (N) availability, to influence soil C flux comprises an important source of this uncertainty, especially for those under-studied ecosystems. By conducting a manipulated CO₂ concentration and N availability experiment on typical alpine grassland (4600 m asl), we combined the five-year in-situ measurement of soil respiration (SR) with an incubation experiment of microbial metabolic efficiency in the lab to explore the response of SR to elevated CO₂ and N availability. The results showed that elevated CO₂ at ambient N conditions and enriched N equally stimulated SR during the experimental period, whereas N supply had no significant effect. Elevated CO₂ enhanced soil dissolved organic C and enzyme activity, while had marginal effects on microbial biomass and C use efficiency (CUE). Strengthened microbial activity dominated SR stimulation under elevated CO₂. Enriched N boosted enzyme activity and microbial CUE. N availability played divergent roles in mediating SR. The negliable regulation of N supply on elevated CO₂ effects on SR was the offset consequences of the negative impacts of enhanced CUE and the positive contribution of heightened enzyme activity. Our findings suggest that rising CO₂ would accelerate soil C cycling of the alpine grassland under various N regimes by stimulating microbial activity instead of lowering microbial metabolic efficiency. Such results are crucial for understanding the role of alpine ecosystems in the global C cycle.

Keywords: Carbon cycling; Elevated CO(2); Enzyme activity; Microbial carbon use efficiency; Nitrogen availability; Soil respiration.