In vitro Arthrographis kalrae biofilm formation: Scanning electron microscopy and cytotoxic analysis

Bianca Dorana de Oliveira Souza; Janneth Josefina Escobar Arcos; Celso Vataru Nakamura; Luciene Airy Nagashima; Ayako Sano; Mario Augusto Ono; Eiko Nakagawa Itano

doi:10.1016/j.jmii.2021.11.002

In vitro Arthrographis kalrae biofilm formation: Scanning electron microscopy and cytotoxic analysis

J Microbiol Immunol Infect. 2022 Oct;55(5):973-976. doi: 10.1016/j.jmii.2021.11.002. Epub 2021 Nov 15.

Authors

Bianca Dorana de Oliveira Souza¹, Janneth Josefina Escobar Arcos², Celso Vataru Nakamura³, Luciene Airy Nagashima⁴, Ayako Sano⁵, Mario Augusto Ono⁶, Eiko Nakagawa Itano⁷

Affiliations

¹ Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil. Electronic address: souza.biancadorana@uel.br.
² Department of Microbiology, State University of Londrina, Londrina, Paraná, Brazil.
³ Department of Microbiology, State University of Maringá, Maringá, Paraná, Brazil.
⁴ Paraná Institute of Technology, Curitiba, Paraná, Brazil.
⁵ Department of Animal Sciences, Ryukyus University, Okinawa, Japan.
⁶ Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil.
⁷ Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil. Electronic address: itano@uel.br.

PMID: 34836818
DOI: 10.1016/j.jmii.2021.11.002

Abstract

To our knowledge, this study represents the first demonstration of Arthrographis kalrae biofilm formation in vitro by scanning electron microscopy and the distinct cytotoxic activity between planktonic and biofilm extracts on RAW 264.7 cell line. Higher activity was observed with biofilm. It could impact host immune response, that require furthers study.

Keywords: Cytotoxicity; Fungi; Macrophage; Virulence factors.

MeSH terms

Ascomycota*
Biofilms
Humans
Microscopy, Electron, Scanning
Plant Extracts

Substances

Plant Extracts

Supplementary concepts

Arthrographis kalrae