Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843CD163 Cell Lines and Primary Porcine Alveolar Macrophages

Xiaoping Wu; Junyang Fang; Qiuping Huang; Xu Chen; Zhongyi Guo; Lingyujia Tian; Enmin Zhou; Jianxin Chen; Yang Mu; Taofeng Du

doi:10.3390/v13112267

Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843^CD163 Cell Lines and Primary Porcine Alveolar Macrophages

Viruses. 2021 Nov 12;13(11):2267. doi: 10.3390/v13112267.

Authors

Affiliations

¹ Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
² Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Abstract

Porcine reproductive and respiratory syndrome (PRRS), a significant viral infectious disease that commonly occurs among farmed pigs, leads to considerable economic losses to the swine industry worldwide. Major vault protein (MVP) is a host factor that induces type Ⅰ interferon (IFN) production. In this study, we evaluated the effect of MVP on PRRSV infection in CRL2843^CD163 cell lines and porcine alveolar macrophages (PAMs). Our results showed that MVP expression was downregulated by PRRSV infection. Adenoviral overexpression of MVP inhibited PRRSV replication, whereas the siRNA knockdown of MVP promoted PRRSV replication. In addition, MVP knockdown has an adverse effect on the inhibitive role of MVP overexpression on PRRSV replication. Moreover, MVP could induce the expression of type Ⅰ IFNs and IFN-stimulated gene 15 (ISG15) in PRRSV-infected PAMs. Based on these results, MVP may be a potential molecular target of drugs for the effective prevention and treatment of PRRSV infection.

Keywords: MVP; PRRSV; antiviral; host factor; type Ⅰ interferon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Interferon Type I / genetics
Interferon Type I / metabolism
Macrophages, Alveolar / metabolism
Macrophages, Alveolar / virology*
Porcine respiratory and reproductive syndrome virus / physiology*
Swine
Vault Ribonucleoprotein Particles / genetics
Vault Ribonucleoprotein Particles / metabolism*
Virus Replication

Substances

Interferon Type I
Vault Ribonucleoprotein Particles
major vault protein