CRISPR Screening: Molecular Tools for Studying Virus-Host Interactions

Viruses. 2021 Nov 11;13(11):2258. doi: 10.3390/v13112258.

Abstract

CRISPR/Cas is a powerful tool for studying the role of genes in viral infections. The invention of CRISPR screening technologies has made it possible to untangle complex interactions between the host and viral agents. Moreover, whole-genome and pathway-specific CRISPR screens have facilitated identification of novel drug candidates for treating viral infections. In this review, we highlight recent developments in the fields of CRISPR/Cas with a focus on the use of CRISPR screens for studying viral infections and identifying new candidate genes to aid development of antivirals.

Keywords: COVID-19; Cas12; Cas9; HAV; HIV; SARS-CoV-2; ZIKV; hepatitis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • CRISPR-Cas Systems*
  • Drug Discovery
  • Genetic Techniques*
  • Genome-Wide Association Study / methods*
  • High-Throughput Screening Assays / methods*
  • Host Microbial Interactions
  • Humans
  • Virus Diseases / genetics*
  • Virus Diseases / virology*
  • Viruses / genetics*