A Novel Distinct Genetic Variant of Tomato Torrado Virus with Substantially Shorter RNA1-Specific 3'Untranslated Region (3'UTR)

Marta Budziszewska; Przemysław Wieczorek

doi:10.3390/plants10112454

A Novel Distinct Genetic Variant of Tomato Torrado Virus with Substantially Shorter RNA1-Specific 3'Untranslated Region (3'UTR)

Plants (Basel). 2021 Nov 13;10(11):2454. doi: 10.3390/plants10112454.

Authors

Marta Budziszewska¹, Przemysław Wieczorek¹

Affiliation

¹ Department of Molecular Biology and Biotechnology, Institute of Plant Protection-National Research Institute, Wegorka 20 Street, 60-318 Poznan, Poland.

Abstract

Tomato torrado virus (ToTV) induces severe systemic necrosis in Solanum lycopersicum. This work aimed at describing the genetic variability of necrosis-inducing ToTV-Wal'17 collected in 2017, derived from the ToTV-Wal'03 after long-term passages in plants. Sequence analyses of the ToTV-Wal'17 indicated twenty-eight single nucleotide substitutions in coding sequence of both RNAs, twelve of which resulted in amino acid changes in viral polyproteins. Moreover the sequencing data revealed that the 3'UTR of ToTV-Wal'17 RNA1 was 394 nts shorter in comparison to Wal'03. The performed sequence analyses revealed that 3'UTR of RNA1 of ToTV-Wal'17 is the most divergent across all previously described European isolates.

Keywords: 3’untranslated regions; genetic variability; tandem repeats; tomato torrado virus.

Grants and funding

2016/21/D/NZ9/02468 and 2016/21/D/NZ9/02478/National Science Center