Identification and antiviral effect of Cherry Valley duck IRF4

Xinyu Zhai; Tianqi Hong; Tingting Zhang; Bin Xing; Jinchao Wang; Xiuyuan Wang; Runchun Miao; Tianxu Li; Liangmeng Wei

doi:10.1016/j.psj.2021.101560

Identification and antiviral effect of Cherry Valley duck IRF4

Poult Sci. 2022 Jan;101(1):101560. doi: 10.1016/j.psj.2021.101560. Epub 2021 Oct 20.

Authors

Xinyu Zhai¹, Tianqi Hong¹, Tingting Zhang², Bin Xing¹, Jinchao Wang¹, Xiuyuan Wang¹, Runchun Miao¹, Tianxu Li¹, Liangmeng Wei³

Affiliations

¹ Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City, 271018, Shandong Province, China.
² Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, College of Basic Medical Sciences, Shandong First Medical University, Tai'an City 271000, Shandong Province, China.
³ Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City, 271018, Shandong Province, China; Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, College of Basic Medical Sciences, Shandong First Medical University, Tai'an City 271000, Shandong Province, China. Electronic address: lmwei@sdau.edu.cn.

Abstract

Interferon regulatory factor 4 (IRF4) is a multifunctional transcription factor that plays an important regulatory role in the interferon (IFN) signaling. IRF4 participates in the process of antivirus, Th cell differentiation and B cell maturation by regulating the expression of IFN and some lymphokines. In this study, Cherry Valley duck IRF4 (duIRF4) was cloned and its cDNA was analyzed. Expression of duIRF4 in a wide variety of tissues and changes in duIRF4 expression due to viral infection also was detected by quantitative real-time PCR. The results show that duIRF4 contains 1,341 bp of ORF encoding a protein with 446 amino acids and contains 3 domains: DNA-binding domain (DBD), IRF-association domain (IAD) and nuclear localization signal (NLS). Quantitative real-time PCR analysis showed that duIRF4 was evenly expressed in all tissues examined, with the highest expression in the spleen, followed by the bursa of Fabricius, and lower in the skin and brain. In addition, expression of duIRF4 in the brain and spleen was significantly upregulated after being infected by duck plague virus, duck Tembusu virus, and novel duck reovirus. These data suggest that duIRF4 may be involved in innate immune response.

Keywords: Cherry Valley duck; IRF4; cloning; innate immunity.

MeSH terms

Animals
Antiviral Restriction Factors / immunology*
Ducks / immunology*
Interferon Regulatory Factors* / immunology
Signal Transduction

Substances

Antiviral Restriction Factors
Interferon Regulatory Factors