Heptapeptide Isolated from Isochrysis zhanjiangensis Exhibited Anti-Photoaging Potential via MAPK/AP-1/MMP Pathway and Anti-Apoptosis in UVB-Irradiated HaCaT Cells

Mar Drugs. 2021 Nov 9;19(11):626. doi: 10.3390/md19110626.

Abstract

Marine microalgae can be used as sustainable protein sources in many fields with positive effects on human and animal health. DAPTMGY is a heptapeptide isolated from Isochrysis zhanjiangensis which is a microalga. In this study, we evaluated its anti-photoaging properties and mechanism of action in human immortalized keratinocytes cells (HaCaT). The results showed that DAPTMGY scavenged reactive oxygen species (ROS) and increase the level of endogenous antioxidants. In addition, through the exploration of its mechanism, it was determined that DAPIMGY exerted anti-photoaging effects. Specifically, the heptapeptide inhibits UVB-induced apoptosis through down-regulation of p53, caspase-8, caspase-3 and Bax and up-regulation of Bcl-2. Thus, DAPTMGY, isolated from I. zhanjiangensis, exhibits protective effects against UVB-induced damage.

Keywords: MMPs; UVB; apoptosis; heptapeptide; photoaging.

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Apoptosis / drug effects
  • Aquatic Organisms
  • HaCaT Cells / drug effects
  • Haptophyta*
  • Humans
  • MAP Kinase Signaling System / drug effects
  • Matrix Metalloproteinases / metabolism
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Skin Aging / drug effects
  • Transcription Factor AP-1 / metabolism
  • Ultraviolet Rays

Substances

  • Antioxidants
  • Peptides
  • Transcription Factor AP-1
  • Matrix Metalloproteinases