Objective: To investigate the therapeutic effects of Biejia Yugan Granule on hepatic fibrosis caused by compound factors in rats and its effect on TGF-β1/Smads signaling pathway. Methods: SD rats were randomly divided into blank control group, model control group, colchicine group, Biejia Yugan Granule low, medium and high dose (1.85, 3.70, 7.40 g/kg) groups (n= 8 in each group). The rat model of hepatic fibrosis was established by treating with 5% alcohol 15 ml/kg (ig) everyday and injecting with 40% carbon tetrachloride (sc) twice a week for 42 days. The effects of Biejia Yugan Granule on liver function, liver index and water content, serum hepatic fibrosis related indicators, key proteins and gene expression of TGF-β1/Smads signaling pathway in rats were observed. Results: Biejia Yugan Granule at the doses of 1.85, 3.70 and 7.40 g/kg could decrease the serum levels of ALT, AST, ALP and HA, PCⅢ, C-Ⅳ, LN significantly, reduce the water content of liver tissue leads to the decrease of liver index, regulate the liver tissue TGF-β1, Smad3 mRNA and Smad7 mRNA expressions. Conclusion: Biejia Yugan Granule has obvious effects of reducing enzyme and protecting liver and inhibiting hepatic fibrosis, and inhibiting TGF-β1/Smads signaling pathway is one of its mechanisms of anti-hepatic fibrosis.
目的: 探讨鳖甲育肝颗粒对复合因素所致肝纤维化大鼠的治疗作用及其对TGF-β1/Smads信号通路的影响。方法: 将SD大鼠随机分为空白对照组、模型组、秋水仙碱组、鳖甲育肝颗粒各剂量组(1.85、3.70、7.40 g/kg, n=8),通过每天灌胃5%乙醇15 ml/kg的基础及每周皮下注射40%四氯化碳2次复制大鼠肝纤维化模型,连续42 d,观测鳖甲育肝颗粒对大鼠肝功能、肝指数及其含水量、血清肝纤维化相关指标,测定TGF-β1/Smads信号通路关键蛋白及基因表达的影响。结果: 与空白对照组比较,模型组大鼠血清ALT、AST、ALP、HA、PCⅢ、C-Ⅳ、LN活性,肝组织含水量及肝指数、TGF-β1、Smad3 mRNA与Smad7 mRNA表达均显著升高(P＜0.01)。与模型组比较,秋水仙碱不同剂量鳖甲育肝颗粒干预组大鼠上述血清肝功能及肝纤维化相关指标,肝组织含水量及肝指数,TGF-β1及Smad3 mRNA表达显著下降(P＜0.01),而Smad7 mRNA表达均显著升高,(P＜0.01)。结论: 鳖甲育肝颗粒具有明显的降酶保肝、抗肝纤维化的作用,而抑制TGF-β1/Smads信号通路是其抗肝纤维化的作用机制之一。.
Keywords: Biejia Yugan Granule (BYG); TGF-β1/Smads signaling pathway; hepatic fibrosis (HF); rat; therapeutic effect.