Blood-gas vs. Central-Laboratory analyzers: interchangeability and reference intervals for sodium, potassium, glucose, lactate and hemoglobin

Kocijancic Marija; Kraus Frank Bernhard; Ludwig-Kraus Beatrice

doi:10.1016/j.heliyon.2021.e08302

Blood-gas vs. Central-Laboratory analyzers: interchangeability and reference intervals for sodium, potassium, glucose, lactate and hemoglobin

Heliyon. 2021 Oct 30;7(11):e08302. doi: 10.1016/j.heliyon.2021.e08302. eCollection 2021 Nov.

Authors

Kocijancic Marija¹, Kraus Frank Bernhard^{1

2}, Ludwig-Kraus Beatrice¹

Affiliations

¹ Central Laboratory, Department for Laboratory Medicine, University Hospital Halle, Halle (Saale), Germany.
² Department of Biology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.

Abstract

Background: Blood-Gas Analyzers (BGAs) are commonly used in parallel with central laboratory analyzers (CLAs). Given the often-divergent results between BGAs and CLAs this study aims to: 1. Determine whether the measurements of potassium (K), sodium (Na), glucose (Glu), lactate (Lact) and total hemoglobin (ctHb) on BGAs and CLAs are interchangeable; 2. Establish reference intervals (RIs) for both analyzer systems using an indirect statistical approach.

Methods: During a one-year study period K, Na, Glu, Lact and ctHb measurements from 500 arterial blood samples, measured on ABL 90 FLEX BGAs were compared with corresponding venous samples measured on Roche c8000 and Sysmex XN-9000 analyzers. Interchangeability of methods was tested based on the Acceptable Change Limit, Total Change Limit and the guidelines published by the German Medical Association for quality assurance in medical laboratories criteria. Indirect RIs were estimated based on all routine analysis data using the software Reference Limit Estimator (RLE).

Results: With the exception of Na, the BGAs differed significantly from the CLAs for the tested analytes (P < 0.001) but, with the exception of ctHb, did meet the interchangeability criteria. For K, Na, Gluc and ctHb the reference intervals obtained with RLE did not differ statistically between the analyzer systems.

Conclusion: The interchangeability criteria were met for Na, K and Gluc and Lact. The indirect RIs obtained with RLE, are comparable between two systems for Na, K, Gluc an ctHb. Lact differed significantly in the lower reference limit between the BGAs and CLAs. The simultaneous use of both analyzing systems is thus only advisable for Na, K and Gluc.

Keywords: Blood-gas analyzers; Central-laboratory analyzers; Interchangeability; POCT; Reference intervals.