Effects of ocean acidification on the biochemistry, physiology and parental transfer of Ampelisca brevicornis (Costa, 1853)

Abstract

Ocean acidification (OA) has received more attention in the marine research community in recent years than any other topic. Excess carbon dioxide makes the ocean more acidic, threatening marine ecosystems. There has been little research on the impact of OA on crustaceans, particularly on their physiological and potential ecosystem-level consequences. Thus, we investigated the impacts of OA on the physiological and biochemical characteristics of the estuarine amphipod Ampelisca brevicornis. Ovigerous amphipods were harvested from nature and maintained in the laboratory to produce juveniles, which were then further reared to obtain the mature adults (F0) and successive offspring (F1). For this study, four pH treatments (pH 8.1, 7.5, 7.0, and 6.5) mimicking future OA were evaluated to understand the physiological and biochemical effects on the organisms. The findings of this study suggest that A. brevicornis is more vulnerable to OA than was previously established in short-term trials. The survival was significantly reduced as pH decreased over time and a significant interaction between pH and time was observed. Survival was higher in F1 than in F0 juveniles and vice versa in terms of growth. Animal's physiological responses such as growth, burrowing behavior, locomotor activity, swimming speed, ventilation rate and reproductive performance were negatively influenced by acidification. These physiological characteristics can be linked to the oxidative stress induced by global change conditions because excess of free radicals degrade cell functioning, affecting species' biochemical and physiological performance. These alterations may have long-term negative impacts, with ecological consequences. The results of this study provide baseline information regarding the effect of OA on this keystone crustacean that may be useful in simulating the impacts of OA to develop different conceptual models for a better understanding of the consequences and implications of climate change in the future for managing marine ecosystems.

Keywords: Ampelisca brevicornis; Biochemical responses; Multigeneration; Ocean acidification; Parental transfer; Physiological.