Glucose oxidase@zinc-doped zeolitic imidazolate framework-67 as an effective cascade catalyst for one-step chemiluminescence sensing of glucose

Abstract

A chemiluminescence (CL) sensor was constructed for the one-step determination of glucose. Glucose oxidase (GOx) was successfully encapsulated into Zn-doped zeolitic imidazolate framework-67 (Zn-ZIF-67) via a simple one-pot strategy. The as-prepared GOx@Zn-ZIF-67 nanocomposite can trigger cascade reactions of glucose oxidation to generate H₂O₂ and H₂O₂-mediated luminol reaction to give an intense CL emission. The sensor responds linearly to glucose in the 20.0-400.0 μmol·L^-1 range with a limit of detection (LOD) of 4.7 μmol·L^-1. Eleven replicated measurements of 200.0 μmol·L^-1 glucose solution gives a relative standard deviation (RSD) of 1.7%. The sensor exhibits good selectivity and stability and was successfully applied to the determination of glucose in real human serum samples. Schematic representation of one-step determination of serum glucose with GOx@Zn-ZIF-67 nanocomposite triggering cascade reactions between luminol and glucose.

Keywords: Cascade catalytic reaction; Chemiluminescence; Glucose determination; Glucose oxidase; Zn-doped zeolitic imidazolate framework-67.