In the research of resistant aging, the concentration of Growth differentiation factor-11(GDF11) is an indispensable parameter. So the accurate detection of GDF11 is very important in life science and medical cosmetology. Hereby, we proposed and demonstrated a simple method to detect low concentration GDF11 by using fiber surface plasmon resonance (SPR) sensor decorated with two-dimension (2D) material Ti3C2-MXene and gold nanosphere. The sensitivity of the fiber SPR sensor was increased to be 4804.64nm/RIU. After functionalized with GDF11 antibody, the fiber SPR sensor could specifically recognize GDF11, and the limit of detection (LOD) can reach 0.577pg/L which is 100 times lower than that of single-molecule ELISA method.