Molecular identification and function characterization of four alternative splice variants of trim25 in Japanese flounder (Paralichthys olivaceus)

Yanan Guo; Zhaodi Sun; Yitong Zhang; Guixing Wang; Zhongwei He; Yufeng Liu; Yuqin Ren; Yufen Wang; Yuanshuai Fu; Jilun Hou

doi:10.1016/j.fsi.2021.11.022

Molecular identification and function characterization of four alternative splice variants of trim25 in Japanese flounder (Paralichthys olivaceus)

Fish Shellfish Immunol. 2022 Jan:120:142-154. doi: 10.1016/j.fsi.2021.11.022. Epub 2021 Nov 20.

Authors

Yanan Guo¹, Zhaodi Sun¹, Yitong Zhang², Guixing Wang², Zhongwei He², Yufeng Liu², Yuqin Ren², Yufen Wang², Yuanshuai Fu³, Jilun Hou⁴

Affiliations

¹ Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Genetics, Shanghai, 201306, China; Laboratory of Cell and Molecular Biology, Shanghai Ocean University, Shanghai, 201306, China.
² Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China.
³ Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Genetics, Shanghai, 201306, China; Laboratory of Cell and Molecular Biology, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: ysfu@shou.edu.cn.
⁴ Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China. Electronic address: houjl@cafs.ac.cn.

PMID: 34808358
DOI: 10.1016/j.fsi.2021.11.022

Abstract

Trim25 is a member of Tripartite Motif (TRIM) family. Previous studies report that trim25 modulates antiviral activity by activating RIG-I. In this study we explored the four alternative splicing (AS) variants X1-X4 of Japanese flounder trim25. The sequences of the AS variants were highly conserved. Expression levels of trim25 X1-X4 were increased after 12 h of poly I:C treatment in vitro. In vivo expression of X2-X4 in liver, kidney (except X2) and blood was significantly up-regulated in early stages of poly I:C treatment. Subcellular localization analysis showed that Trim25 X1-X4 were distributed in different cellular organelles. The recombinant vector pcDNA3.1-Trim25 X1-X4 were successfully overexpressed in Flounder cells and the samples were collected. Expression patterns of RIG-I pathway genes dhx58, traf6, traf2, nfkbia and il-8 were explored in vitro and in vivo after poly I:C treatment, as well as overexpressed samples. The findings of this study imply that AS variants of trim25 confer antiviral activity in Japanese flounder by modulating innate immune response.

Keywords: Alternative splicing; Innate immune response; Japanese flounder; trim25.

MeSH terms

Alternative Splicing*
Animals
Fish Proteins* / genetics
Flounder* / genetics
Flounder* / immunology
Immunity, Innate*
Poly I-C / pharmacology
Tripartite Motif Proteins / genetics*

Substances

Fish Proteins
Tripartite Motif Proteins
Poly I-C