Experimental validation under controlled conditions of real time PCR to quantify arbuscular mycorrhizal colonization in root

Bruna Arruda; Yasmin Florentino Rodrigues; Wilfrand Ferney Bejarano Herrera; Agnès Robin; Simone Raposo Cotta; Fernando Dini Andreote

doi:10.1016/j.mimet.2021.106382

Experimental validation under controlled conditions of real time PCR to quantify arbuscular mycorrhizal colonization in root

J Microbiol Methods. 2022 Jan:192:106382. doi: 10.1016/j.mimet.2021.106382. Epub 2021 Nov 20.

Authors

Bruna Arruda¹, Yasmin Florentino Rodrigues², Wilfrand Ferney Bejarano Herrera³, Agnès Robin⁴, Simone Raposo Cotta⁵, Fernando Dini Andreote⁶

Affiliations

¹ Department of Soil Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Piracicaba, Brazil.. Electronic address: barruda@alumni.usp.br.
² Department of Soil Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Piracicaba, Brazil.. Electronic address: yasmin-florentino@usp.br.
³ Colombian Corporation for Agricultural Research (Agrosavia), Pasto, Colombia. Electronic address: wbejarano@agrosavia.co.
⁴ Department of Soil Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Piracicaba, Brazil.; CIRAD, UMR Eco&Sols, Piracicaba, Brazil; Eco&Sols, Université Montpellier, CIRAD, INRAE, IRD, Montpellier, France. Electronic address: agnes.robin@cirad.fr.
⁵ Department of Soil Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Piracicaba, Brazil.; University of São Paulo, Center for Nuclear Energy in Agriculture, Piracicaba, Brazil.
⁶ Department of Soil Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Piracicaba, Brazil.. Electronic address: fdandreo@usp.br.

PMID: 34808146
DOI: 10.1016/j.mimet.2021.106382

Abstract

Mycorrhizal colonization of roots is traditionally evaluated by empirical methods, such as root microscopy. We compared this method with data from using a real time PCR technique, and determined the correlation between methods, indicating particularities of a promising system for a quick and accurate molecular diagnostic of arbuscular mycorrhization.

Keywords: Brachiaria; Crotalaria; Glomeromycota; Microscopy; qPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brachiaria / microbiology
Crotalaria / microbiology
Fungi / genetics
Fungi / growth & development*
Mycorrhizae / growth & development*
Plant Roots / microbiology
Real-Time Polymerase Chain Reaction
Ribosome Subunits, Large / genetics
Soil Microbiology
Spores, Fungal / growth & development*

Supplementary concepts

Acaulospora colombiana
Dentiscutata heterogama
Rhizophagus clarus