Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures

Katharina Bach; Birgit Edel; Steffen Höring; Lucie Bartoničkova; Stefan Glöckner; Bettina Löffler; Christina Bahrs; Jürgen Rödel

doi:10.1007/s10096-021-04383-3

Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures

Eur J Clin Microbiol Infect Dis. 2022 Mar;41(3):489-494. doi: 10.1007/s10096-021-04383-3. Epub 2021 Nov 22.

Authors

Katharina Bach¹, Birgit Edel¹, Steffen Höring², Lucie Bartoničkova¹, Stefan Glöckner¹, Bettina Löffler¹, Christina Bahrs^{3

4}, Jürgen Rödel⁵

Affiliations

¹ Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
² Division of Infection Control and Infectious Diseases, RWTH Aachen University Hospital, Aachen, Germany.
³ Institute for Infectious Diseases and Infection Control, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
⁴ Division of Infectious Diseases and Tropical Medicine, Department of Medicine I, Medical University of Vienna, Vienna, Austria.
⁵ Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, Jena, Germany. juergen.roedel@med.uni-jena.de.

Abstract

The LAMP-based eazyplex® BloodScreen GN was evaluated for the detection of frequent Gram-negatives directly from positive blood culture (BC) bottles. A total of 449 BCs were analyzed. Sensitivities and specificities were 100% and 100% for Escherichia coli, 95.7% and 100% for Klebsiella pneumoniae, 100% and 100% for bla_CTX-M, 100% and 100% for Klebsiella oxytoca, 100% and 99% for Proteus mirabilis, and 100% and 99.8% for Pseudomonas aeruginosa, respectively. The time to result ranged from 8 to 16 min, plus about 6 min for sample preparation. The eazyplex® BloodScreen GN is a reliable molecular assay for rapid BC testing.

Keywords: Blood culture; Gram-negative bacilli; LAMP; Rapid diagnostics; Sepsis.

MeSH terms

Bacteremia* / diagnosis
Blood Culture
Escherichia coli Infections*
Gram-Negative Bacteria / genetics
Humans