Prevalence of Newcastle Disease Virus in Commercial and Backyard Poultry in Haryana, India

Vinay G Joshi; Deepika Chaudhary; Nitish Bansal; Renu Singh; Sushila Maan; Nand K Mahajan; Chintu Ravishankar; Niranjana Sahoo; Sunil K Mor; Jessica Radzio-Basu; Catherine M Herzog; Vivek Kapur; Parveen Goel; Naresh Jindal; Sagar M Goyal

doi:10.3389/fvets.2021.725232

Prevalence of Newcastle Disease Virus in Commercial and Backyard Poultry in Haryana, India

Front Vet Sci. 2021 Nov 4:8:725232. doi: 10.3389/fvets.2021.725232. eCollection 2021.

Authors

Vinay G Joshi¹, Deepika Chaudhary², Nitish Bansal², Renu Singh², Sushila Maan¹, Nand K Mahajan², Chintu Ravishankar³, Niranjana Sahoo⁴, Sunil K Mor⁵, Jessica Radzio-Basu^{6

7}, Catherine M Herzog⁶, Vivek Kapur^{6

7}, Parveen Goel⁸, Naresh Jindal², Sagar M Goyal⁵

Affiliations

¹ Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
² Department of Veterinary Public Health & Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
³ Department of Veterinary Microbiology, Kerala Veterinary and Animal Sciences University, Pookode, India.
⁴ College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, India.
⁵ Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, MN, United States.
⁶ The Huck Institute of the Life Sciences, University Park, PA, United States.
⁷ Department of Animal Science, The Pennsylvania State University, University Park, PA, United States.
⁸ Directorate of Research, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.

Abstract

Newcastle disease virus (NDV) causes Newcastle disease (ND) in poultry. The ND is a highly contagious disease, which is endemic in several countries despite regular vaccination with live or killed vaccines. Studies on NDV in India are mostly targeted toward its detection and characterization from disease outbreaks. A surveillance study was undertaken to determine NDV prevalence throughout the state of Haryana from March 2018 to March 2020 using a stratified sampling scheme. The state was divided into three different zones and a total of 4,001 choanal swab samples were collected from backyard poultry, commercial broilers, and layers. These samples were tested for the M gene of NDV using real-time RT-PCR. Of the 4,001 samples tested, 392 were positive (9.8% apparent prevalence; 95% CI: 8.9-10.8%) for the M gene. Of these 392 M gene positive samples, 35 (8.9%; 95% CI: 6.4-12.3%) were found to be positive based on F gene real-time RT-PCR. Circulation of NDV in commercial and backyard poultry highlights the importance of surveillance studies even in apparently healthy flocks. The information generated in this study should contribute to better understanding of NDV epidemiology in India and may help formulate appropriate disease control strategies for commercial and backyard birds.

Keywords: Newcastle disease virus; RT-PCR; backyard birds; commercial poultry; surveillance.