Sensitive and selective detection of target analyte is very important in many fields such as commodity inspection and quality monitoring. In this work, based on the principle of competitive immunoassay, surface-enhanced Raman spectroscopy (SERS) was used to establish a rapid and highly sensitive method for the detection of trace amounts of bisphenol A in water. Here, Raman molecule 5,5-dithiobis-2-nitrobenzoic acid and anti-BPA antibody were conjugated with Au (core)@Ag (shell) nanoparticle to serve as SERS nanoprobe. After the SERS nanoprobe is combined with the substance to be tested, it uses the siphon effect to pass through the test line and the charging line on the test strip. And the Raman test was performed on the T line with a Raman spectrometer. The detection limitation was 0.1 pg/mL. Compared with the reported gas chromatography, liquid chromatography, fluorescence analysis, and other detection methods, SERS ICA does not demand complicated sample preparation procedures, and has the advantages of simple detection methods, quick results, High sensitivity, good specificity, and low technical demands for laboratory environment and testers. In addition, Raman spectrometers have gradually developed to be portable, making it easier to meet the needs of on-site rapid and highly sensitive detection, and will show broad prospects for applications in the fields of biomedical diagnosis and food safety monitoring.
Keywords: Bisphenol A; Immunochromatographic; Surface-enhanced Raman spectroscopy; Trace detection.
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