Strong and Specific Recognition of CAG/CTG Repeat DNA (5'-dWGCWGCW-3') by a Cyclic Pyrrole-Imidazole Polyamide

Chembiochem. 2022 Jan 19;23(2):e202100533. doi: 10.1002/cbic.202100533. Epub 2021 Nov 18.

Abstract

Abnormally expanded CAG/CTG repeat DNA sequences lead to a variety of neurological diseases, such as Huntington's disease. Here, we synthesized a cyclic pyrrole-imidazole polyamide (cPIP), which can bind to the minor groove of the CAG/CTG DNA sequence. The double-stranded DNA melting temperature (Tm ) and surface plasmon resonance assays revealed the high binding affinity of the cPIP. In addition, next-generation sequencing showed that the cPIP had high specificity for its target DNA sequence.

Keywords: CAG/CTG repeats; DNA recognition; pyrrole-imidazole polyamides; triplet repeats.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • Imidazoles / chemistry*
  • Nylons / chemistry*
  • Pyrroles / chemistry*
  • Repetitive Sequences, Nucleic Acid*
  • Surface Plasmon Resonance

Substances

  • Imidazoles
  • Nylons
  • Pyrroles
  • DNA