Serotypes, Virulence-Associated Factors, and Antimicrobial Resistance of Streptococcus suis Isolates Recovered From Sick and Healthy Pigs Determined by Whole-Genome Sequencing

Maverick Aradanas; Zvonimir Poljak; Nahuel Fittipaldi; Nicole Ricker; Abdolvahab Farzan

doi:10.3389/fvets.2021.742345

Serotypes, Virulence-Associated Factors, and Antimicrobial Resistance of Streptococcus suis Isolates Recovered From Sick and Healthy Pigs Determined by Whole-Genome Sequencing

Front Vet Sci. 2021 Nov 2:8:742345. doi: 10.3389/fvets.2021.742345. eCollection 2021.

Authors

Maverick Aradanas¹, Zvonimir Poljak², Nahuel Fittipaldi³, Nicole Ricker¹, Abdolvahab Farzan^{1

2}

Affiliations

¹ Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
² Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
³ Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC, Canada.

Abstract

Streptococcus suis is ubiquitous in swine, and yet, only a small percentage of pigs become clinically ill. The objective of this study was to describe the distribution of serotypes, virulence-associated factor (VAF), and antimicrobial resistance (AMR) genes in S. suis isolates recovered from systemic (blood, meninges, spleen, and lymph node) and non-systemic (tonsil, nasal cavities, ileum, and rectum) sites of sick and healthy pigs using whole-genome sequencing. In total, 273 S. suis isolates recovered from 112 pigs (47 isolates from systemic and 136 from non-systemic sites of 65 sick pigs; 90 isolates from non-systemic sites of 47 healthy pigs) on 17 Ontario farms were subjected to whole-genome sequencing. Using in silico typing, 21 serotypes were identified with serotypes 9 (13.9%) and 2 (8.4%) as the most frequent serotypes, whereas 53 (19.4%) isolates remained untypable. The relative frequency of VAF genes in isolates from systemic (Kruskal-Wallis, p < 0.001) and non-systemic (Kruskal-Wallis, p < 0.001) sites in sick pigs was higher compared with isolates from non-systemic sites in healthy pigs. Although many VAF genes were abundant in all isolates, three genes, including dltA [Fisher's test (FT), p < 0.001], luxS (FT, p = 0.01), and troA (FT, p = 0.02), were more prevalent in isolates recovered from systemic sites compared with non-systemic sites of pigs. Among the isolates, 98% had at least one AMR gene, and 79% had genes associated with at least four drug classes. The most frequently detected AMR genes were tetO conferring resistance to tetracycline and ermB conferring resistance to macrolide, lincosamide, and streptogramin. The wide distribution of VAFs genes in S. suis isolates in this study suggests that other host and environmental factors may contribute to S. suis disease development.

Keywords: Streptococcus suis; antimicrobial resistance; in silico serotyping; swine; virulence factors; whole genome sequencing.