C/EBPZ modulates the differentiation and proliferation of preadipocytes

Yuechan Chen; Lingyu Gao; Tao Lin; Xuelian Pei; Qin Gao; Jingjing Chen; Yi Zhang; Xiangqi Wu; Zequan Li; Zhiwei Zhang

doi:10.1038/s41366-021-01020-z

C/EBPZ modulates the differentiation and proliferation of preadipocytes

Int J Obes (Lond). 2022 Mar;46(3):523-534. doi: 10.1038/s41366-021-01020-z. Epub 2021 Nov 17.

Authors

Yuechan Chen^#^{1

2}, Lingyu Gao^#^{1

2}, Tao Lin^{1

2}, Xuelian Pei^{1

2

3}, Qin Gao^{1

2}, Jingjing Chen^{1

2}, Yi Zhang^{1

2}, Xiangqi Wu^{1

2}, Zequan Li^{1

2}, Zhiwei Zhang^{4

5

6}

Affiliations

¹ NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases of First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.
² Key Laboratory of Xinjiang Endemic and Ethnic Diseases of Ministry of Education, School of Medicine, Shihezi University, Shihezi, China.
³ Department of Histology and Embryology, School of Medicine, Shihezi University, Shihezi, China.
⁴ NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases of First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China. zzwneau@163.com.
⁵ Key Laboratory of Xinjiang Endemic and Ethnic Diseases of Ministry of Education, School of Medicine, Shihezi University, Shihezi, China. zzwneau@163.com.
⁶ Department of Histology and Embryology, School of Medicine, Shihezi University, Shihezi, China. zzwneau@163.com.

^# Contributed equally.

Abstract

Background/objectives: This study investigated the functions of CCAAT/enhancer-binding protein zeta (C/EBPZ; Gene ID: 10153) in adipose tissue.

Subjects/methods: Bioinformatics analysis were used to study the expression pattern of C/EBPZ in human adipose tissue. The expression and function of C/EBPZ in adipose tissue were further studied using chicken as animal model in vivo and in vitro.

Results: The human C/EBPZ transcripts were greater and more stable in subcutaneous adipose tissue than in visceral adipose tissue (P < 0.01), and they were increased with age in adipose tissue (P < 0.05). In addition, the chicken C/EBPZ transcripts (C/EBPZ /ACTB) of visceral (abdominal) adipose tissue were significantly different between fat and lean broilers and decreased with age during development (P < 0.01). RNA-seq analysis showed that the C/EBPZ overexpression associated with adipose tissue development and DNA replication in chicken preadipocytes (P < 0.05). Additionally, overexpression of chicken C/EBPZ inhibited preadipocytes differentiation and promoted preadipoytes proliferation in vitro (P < 0.05). In addition, C/EBPZ overexpression suppressed the promoter activities of PPARγ, C/EBPα, FASN and LPL, and promoted the promoter activities of GATA2 and FABP4 in chicken preadipocytes (P < 0.05).

Conclusions: C/EBPZ modulated the differentiation and proliferation of preadipocytes, and it might be a new negative regulator of adipogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes* / metabolism
Adipogenesis / genetics
Animals
CCAAT-Enhancer-Binding Protein-alpha / genetics
CCAAT-Enhancer-Binding Protein-alpha / metabolism
Cell Differentiation
Cell Proliferation
Chickens* / genetics
Chickens* / metabolism
PPAR gamma / metabolism

Substances

CCAAT-Enhancer-Binding Protein-alpha
PPAR gamma