BAIAP2L2 promotes the proliferation, migration and invasion of osteosarcoma associated with the Wnt/β-catenin pathway

Hongting Guo; Jing Peng; Juan Hu; Shichuan Chang; Huawen Liu; Hao Luo; Xiaohua Chen; Haiping Tang; Youhao Chen

doi:10.1016/j.jbo.2021.100393

BAIAP2L2 promotes the proliferation, migration and invasion of osteosarcoma associated with the Wnt/β-catenin pathway

J Bone Oncol. 2021 Oct 12:31:100393. doi: 10.1016/j.jbo.2021.100393. eCollection 2021 Dec.

Authors

Hongting Guo¹, Jing Peng², Juan Hu³, Shichuan Chang¹, Huawen Liu¹, Hao Luo⁴, Xiaohua Chen⁴, Haiping Tang², Youhao Chen⁴

Affiliations

¹ Oncologe Department, Chong Qing University Three Gorges Hospital, Chongqing City 404100, PR China.
² Blood Transfusion Department, Chong Qing University Three Gorges Hospital, Chongqing City 404100, PR China.
³ Department of Anesthesiology, Chong Qing University Three Gorges Hospital, Chongqing City 404100, PR China.
⁴ Department of Orthopaedics, Chong Qing University Three Gorges Hospital, Chongqing City 404100, PR China.

Abstract

Background: Osteosarcoma is the most common bone cancer that significantly affects the quality of life of patients. Studies have shown that overexpression of BAIAP2L2 elevates the proliferation and growth of some types of cancer cells. However, the role of BAIAP2L2 in osteosarcoma is unclear. This study aimed to investigate the functions of BAIAP2L2 in the development of osteosarcoma.

Methods: We used immunohistochemical and Western blot analysis to determine the expression levels of endogenic BAIAP2L2 in osteosarcoma cells. Cell counting kit-8 assay and colony formation assay were performed to investigate cell proliferation of tumor cells. Transwell assay was performed to detect cell migration. Flow cytometry assay was used to analyze cell apoptosis. The role of BAIAP2L2 in tumor growth was further explored in vivo.

Results: We found that BAIAP2L2 was significantly upregulated in human osteosarcoma, and inhibition of BAIAP2L2 suppressed the proliferation of osteosarcoma cells. In addition, down-regulation of BAIAP2L2 could lead to osteosarcoma cancer cell apoptosis, inhibit cell migration and invasion, and induce the inactivation of the Wnt/β-catenin pathway. In addition, down-regulation of BAIAP2L2 inhibited tumor growth in vivo.

Conclusion: In conclusion, down-regulation of BAIAP2L2 inhibited the proliferation, migration, and invasion of osteosarcoma associated with the Wnt/β-catenin pathway.

Keywords: BAIAP2L2; Combinational role; Osteosarcoma; Wnt/β-catenin pathway.