Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma

Oncogene. 2022 Jan;41(4):538-549. doi: 10.1038/s41388-021-02114-8. Epub 2021 Nov 16.

Abstract

Although tobacco smoking is a risk factor for lung adenocarcinoma (LUAD), the mechanisms by which tobacco smoking induces LUAD development remain elusive. Histone methylation levels in human bronchial epithelial cells have been reported to increase after exposure to cigarettes. In this study, we explored the mechanisms regulating histone methylation in LUAD in response to smoking. We found that the histone H3K9 methylation reader CBX3 was upregulated in current smokers with LUAD, and that CBX3 overexpression promoted LUAD progression. Functional enrichment analyses revealed that CBX3 regulated the activation of Rho GTPases in LUAD. We also found that by forming a complex with TRIM28, TRIM24, and RBBP4, CBX3 repressed the expression of ARHGAP24 and increased the amount of active Rac1 in LUAD cells. Collectively, these results suggest that smoking associated upregulation of CBX3 promotes LUAD progression by activating the ARHGAP24/Rac1 pathway. Hence, the CBX3/ARHGAP24/Rac1 axis may represent a promising therapeutic target in smoking-induced LUAD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma of Lung / etiology*
  • Adenocarcinoma of Lung / pathology
  • Animals
  • Cell Line, Tumor
  • Chromosomal Proteins, Non-Histone / metabolism*
  • GTPase-Activating Proteins / metabolism*
  • Humans
  • Lung Neoplasms / etiology*
  • Lung Neoplasms / pathology
  • Mice
  • Mice, Nude
  • Tobacco Smoking / adverse effects*
  • Up-Regulation
  • rac1 GTP-Binding Protein / metabolism*

Substances

  • ARHGAP24 protein, mouse
  • Cbx3 protein, mouse
  • Chromosomal Proteins, Non-Histone
  • GTPase-Activating Proteins
  • rac1 GTP-Binding Protein