Dual-response electrochemical electrode for sensitive monitoring of topotecan and mitomycin as anticancer drugs in real samples

Abstract

This research paper employed an innovative electrochemical electrode to simultaneously determine topotecan (TPT) and mitomycin (MMC) as anticancer agents. For this purpose, a novel nanocomposite was synthesized using a hydrothermal procedure. The nanocomposites were characterized using FTIR, STEM, FESEM, mapping analysis, EDX, and XRD methods. The novelty of this work is the successful synthesis of Fe₃O₄ decorated on the surface of CuCo₂S₄ (Fe₃O₄@CuCo₂S₄) nanocomposites showed two separate anodic peaks at 0.8 V for TPT and 1.0 V for MMC with potential separation of 0.2 V. This was enough for the simultaneous electrochemical determination of topotecan and mitomycin on a glassy carbon electrode (GCE), simultaneously. At optimized conditions, the developed electrode exhibited linear responses with TPT and MMC concentration in the ranges of 0.01-0.89 and 0.89-8.95 μM for topotecan and 0.1-19.53 μM for mitomycin. The detection limits were observed as 6.94 nM and 80.00 nM for topotecan and mitomycin, respectively. The fabricated Fe₃O₄@CuCo₂S₄/GCE showed high sensitivity, long-term stability, and repeatability towards the sensing of TPT and MMC simultaneously and can be utilized in real samples. The obtained results confirmed that the fabricated Fe₃O₄@CuCo₂S₄/GCE nanocomposites can be utilize in the simultaneous electrochemical determination of topotecan and mitomycin in real samples.

Keywords: Antineoplastic agent; Fe(3)O(4)@CuCo(2)S(4); Glassy carbon electrode; Mitomycin; Topotecan.