The dust charge of the two species in a binary mixture of particles in a dusty plasma has been measured using the concept of configurational temperature. There, the dust charge and the respective dust charge ratio are determined from the comparison of the instantaneous particle positions and the kinetic temperature. For that purpose, experiments of binary mixtures of melamine-formaldehyde and silica particles have been evaluated. The configurational temperature approach has also been checked against simulations. From these analyses it is found that the charge ratio of the two species can be obtained quite accurately, whereas for the determination of the absolute charge values a good knowledge of the confining potential is required.