Virulence and horizontal transmission of Metarhizium anisopliae by the adults of the greenhouse whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) and the efficacy of oil formulations against its nymphs

Vongai M Paradza; Fathiya M Khamis; Abdullahi A Yusuf; Sevgan Subramanian; Komivi S Akutse

doi:10.1016/j.heliyon.2021.e08277

Virulence and horizontal transmission of Metarhizium anisopliae by the adults of the greenhouse whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) and the efficacy of oil formulations against its nymphs

Heliyon. 2021 Oct 28;7(11):e08277. doi: 10.1016/j.heliyon.2021.e08277. eCollection 2021 Nov.

Authors

Vongai M Paradza^{1

2}, Fathiya M Khamis¹, Abdullahi A Yusuf^{2

3}, Sevgan Subramanian¹, Komivi S Akutse¹

Affiliations

¹ International Centre of Insect Physiology and Ecology (icipe), P.O. Box 30772-00100, Nairobi, Kenya.
² Department of Zoology and Entomology, University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa.
³ Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa.

Abstract

The pathogenicity of dry conidia and fungal suspensions of 16 entomopathogenic fungal isolates (10 Metarhizium anisopliae and six Beauveria bassiana) was evaluated against adults and second instar nymphs of the greenhouse whitefly, Trialeurodes vaporariorum respectively. All the tested isolates were pathogenic to T. vaporariorum and caused mortality of 45-93% against the adults and 24-89% against the nymphs. However, M. anisopliae strains showed higher virulence to both developmental stages as compared to B. bassiana strains. The three most virulent isolates that caused high mortalities in adults were M. anisopliae ICIPE 18, ICIPE 62 and ICIPE 69, with cumulative mortalities of 82, 91 and 93%, and median lethal times (LT₅₀) of 5.20, 5.05 and 4.78 days, respectively. These isolates were further assessed for spore acquisition and retention by the adult insects at 0, 24, 48 and 72 h after exposure to dry conidia spores. There was no significant difference among isolates on their acquisition by the insects, although the effect of time on the number of spores retained by each insect was significant. For M. anisopliae ICIPE 62 and ICIPE 69, spore number was significantly higher immediately after exposure at 0 h than at 24, 48 and 72 h, whereas for M. anisopliae ICIPE 18, the spore number remained constant for all the days. The infected "donor" insects were able to horizontally transmit the acquired spores to uninfected "recipient" insects causing high mortality rates in both donor and recipient groups. Metarhizium anisopliae ICIPE 7, ICIPE 18 and ICIPE 62 were the most virulent isolates against the nymphs in aqueous formulation during the first screening with >80% mortality. However, in 2% (v/v) oil formulations at 1 × 10⁸ conidia/ml, canola formulated ICIPE 62, ICIPE 18 and olive formulated ICIPE 18 were the most effective, resulting in 87.8, 88.1 and 99.4% nymphal mortalities respectively and with lower LT₅₀. Oil formulations significantly enhanced the efficacy and virulence of the isolates against the nymphs compared to aqueous formulations.

Keywords: Conidia acquisition and retention; Entomopathogenic fungus; Inoculum transmission; Trialeurodes vaporariorum; Vegetable oil formulations.