Arabidopsis glutamate receptor GLR3.7 is involved in abscisic acid response

Pei-Yuan Chen; Chun-Yi Hsu; Cheng-En Lee; Ing-Feng Chang

doi:10.1080/15592324.2021.1997513

Arabidopsis glutamate receptor GLR3.7 is involved in abscisic acid response

Plant Signal Behav. 2021 Dec 2;16(12):1997513. doi: 10.1080/15592324.2021.1997513. Epub 2021 Nov 12.

Authors

Pei-Yuan Chen¹, Chun-Yi Hsu¹, Cheng-En Lee¹, Ing-Feng Chang^{1

2

3}

Affiliations

¹ Institute of Plant Biology, National Taiwan University, Taipei, Taiwan.
² Department of Life Science, National Taiwan University, Taipei, Taiwan.
³ Genome and Systems Biology Degree Program, National Taiwan University, Taipei, Taiwan.

Abstract

The ionotropic glutamate receptor (iGluR) plays an important role in neuronal signaling in animal cells. There are at least 20 glutamate receptor-like (GLR) genes in Arabidopsis thaliana. These genes are involved in seed germination, root growth, wounding response, stomata closure, etc. A recent study showed that Arabidopsis clade III glutamate receptor GLR3.7 is involved in salt stress response. We tested whether GLR3.7 is involved in abscisic acid (ABA) response. In the present study, we found that the expression of GLR3.7 was reduced by ABA treatment. Under ABA-treated condition, GLR3.7 overexpression lines exhibited significantly higher seed germination rate at 60, 72 and 84 h under ABA-treated condition. A point mutation in 14-3-3 binding site of GLR3.7 in GLR3.7-S860A overexpression lines exhibited higher seed germination inhibition under ABA-treated conditions. Our results support that GLR3.7 is involved in ABA response in Arabidopsis. In addition, Ser-860 of GLR3.7 appears to be important in ABA response.

Keywords: 14-3-3; Arabidopsis; abscisic acid; calcium; glutamate receptor; salt; seed germination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid* / metabolism
Abscisic Acid* / pharmacology
Arabidopsis Proteins* / genetics
Arabidopsis Proteins* / metabolism
Arabidopsis* / metabolism
Gene Expression Regulation, Plant / genetics
Germination / genetics
Mutation
Receptors, Glutamate* / genetics
Receptors, Glutamate* / metabolism
Seeds / metabolism

Substances

Arabidopsis Proteins
Receptors, Glutamate
Abscisic Acid

Grants and funding

This work was supported by the Ministry of Science and Technology, Taiwan [109-2313-B-002-020]; Ministry of Science and Technology, Taiwan [110-2311-B-002-028]; Ministry of Science and Technology, Taiwan [109-2311-B-002-028].