Genetic Code Expansion Through Quadruplet Codon Decoding

Jiantao Guo; Wei Niu

doi:10.1016/j.jmb.2021.167346

Genetic Code Expansion Through Quadruplet Codon Decoding

J Mol Biol. 2022 Apr 30;434(8):167346. doi: 10.1016/j.jmb.2021.167346. Epub 2021 Nov 8.

Authors

Jiantao Guo¹, Wei Niu²

Affiliations

¹ Department of Chemistry, University of Nebraska-Lincoln, United States; The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, United States. Electronic address: jguo4@unl.edu.
² Department of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, United States; The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, United States.

Abstract

Noncanonical amino acid mutagenesis has emerged as a powerful tool for the study of protein structure and function. While triplet nonsense codons, especially the amber codon, have been widely employed, quadruplet codons have attracted attention for the potential of creating additional blank codons for noncanonical amino acids mutagenesis. In this review, we discuss methodologies and applications of quadruplet codon decoding in genetic code expansion both in vitro and in vivo.

Keywords: +1 frameshift; four-base codon; genetic code; non-canonical amino acids; quadruplet codon.

Publication types

Review

MeSH terms

Amino Acids / genetics
Amino Acids / metabolism
Codon, Terminator / genetics
Genetic Code*
Mutagenesis
Protein Conformation*
RNA, Transfer* / metabolism

Substances

Amino Acids
Codon, Terminator
RNA, Transfer

Grants and funding

R01 GM138623/GM/NIGMS NIH HHS/United States