Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan

Abstract

Strain TUM18999^T was isolated from the skin of a patient with burn wounds in Japan. The strain was successfully cultured at 20-42 °C (optimum, 30-35 °C) in 1.0-4.0% NaCl (w/v) and at pH 5.5-9.5, optimum pH 5.5-8.5. The phylogenetic tree reconstructed using 16S rRNA, gyrB, rpoB and rpoD gene sequences indicated that strain TUM18999^T is closely related to Pseudomonas otitidis MCC10330^T. Although the partial 16S rRNA gene sequence (1412 bp) of TUM18999^T exhibits high similarity to those of Pseudomonas alcaligenes NBRC 14159^T (99.08 %) and Pseudomonas otitidis MCC10330^T (98.51 %), multi-locus sequence analysis using 16S rRNA, gyrB, rpoB and rpoD genes reveals a clear distinction between TUM18999^T and other Pseudomonas species. In addition, an average nucleotide identity >90 % was not observed in the P. aeruginosa group. Moreover, TUM18999^T and P. otitidis can be distinguished based on the minimum inhibitory concentration for carbapenem. Meanwhile, the cellular fatty acids are enriched with C_{18 : 1} ω7c/C_{18 : 1} ω6c (34.35 %), C_{16 : 1} ω7c/C_{16 : 1} ω6c (24.22 %), C_{16 : 0} (19.79 %) and C_{12 : 0} (8.25 %). Based on this evidence, strain TUM18999^T can be defined as representing a novel Pseudomonas species, with the proposed name Pseudomonas tohonis sp. nov. The type strain is TUM18999^T (GTC 22698^T=NCTC 14580^T).

Keywords: Pseudomonas tohonis; average nucleotide identity; gyrB; multi-locus sequence analysis; subclass B3 metallo-β-lactamase.