A flow-cytometry-based protocol using diverse cell types for detecting autoantibodies from human plasma and serum samples

STAR Protoc. 2021 Oct 29;2(4):100924. doi: 10.1016/j.xpro.2021.100924. eCollection 2021 Dec 17.

Abstract

Here, we describe a protocol for cell-based detection of autoantibodies from human plasma and serum samples using a standard flow cytometer. The protocol allows detection of autoantibodies against a wide array of extracellular antigens. Antigen coverage is limited to the cell types tested, and researchers will need to further determine if autoantibody-positive samples correlate with cytotoxic or clinical outcomes. This protocol is less expensive and faster to perform when compared to protein microarrays and requires no prior knowledge of potential targets. For complete details on the use and execution of this protocol, please refer to Wong et al. (2021).

Keywords: Antibody; Cell Biology; Cell-based Assays; Flow Cytometry/Mass Cytometry; Health Sciences.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Autoantibodies / blood*
  • Cell Culture Techniques
  • Cell Line
  • Female
  • Flow Cytometry / methods*
  • Fluorescent Dyes
  • Humans
  • Male
  • Middle Aged
  • Young Adult

Substances

  • Autoantibodies
  • Fluorescent Dyes